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This stage is critical as proper orientation with the tissue is important for exact microscopic evaluation. The mould is filled with melted paraffin and afterwards instantly put on a cooling surface area.With respect for the latter, a lot of laboratories locate it realistic to make use of the hold off functionality on an automatic tissue processor

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This really is especially real for immunohistochemistry wherein 10% NBF has been demonstrated to become a weak fixative (Fig. 2). Some issues with using 10% NBF for a fixative happen to be corrected through the introduction of other experimental tactics for example antigen retrieval (Shi et al. 2001) and using compact amplicons in RT-Q-PCR (Steg et